Labelling of antibodies

Labelling of primary antibodies or other proteins to make a fluorescent probe, enzyme conjugate or biotinylated binding reagent for detecting proteins in tissues, cells, or biochemical assays.

Biotin

Biotinylation is to chemically tag and label antibodies, proteins and peptides with biotin at particular amino acid functional groups to create labeled antibody or other probes for streptavidin affinity binding, purification and detection. Moreover, biotin is substantially smaller than enzyme labels and is therefore less likely to interfere with normal protein function.

Enzyme Conjugates

Certain enzymes have properties that enable them to function as highly sensitive probes for the detection of proteins in tissues, whole cells or lysates. Enzyme labels are considerably larger than biotin and require the addition of a substrate to generate a colorimetric, chemiluminescent or fluorescent signal which can be detected by different approaches. Enzyme labels are widely used because of their multiple types of signal output, signal amplification and the wide selection of enzyme-labeled products, especially antibodies.
Commonly used enzymes include horseradish peroxidase (HRP) and alkaline phosphatase (AP). Multiple commercial substrates are available for HRP and AP that generate colorimetric, chemiluminescent or fluorescent signal outputs.

Fluorescent Probes

Fluorescent molecules, also called fluorophores, respond directly to light and produce a detectable signal. Unlike enzymes or biotin, fluorescent labels do not require additional reagents for detection. This feature makes fluorophores extremely versatile and the new standard in detecting protein location and activation, identifying protein complex formation and conformational changes, and monitoring biological processes in vivo.

Please inquire for quotation.
 
 
SwedClone | Uminova Science Park | Box 7964 | SE-907 19 Umeå, Sweden | Phone: +46 90 15 49 40 / +46 70 655 9440 | E-mail: info@swedclone.com
site created and hosted by skandiaweb