Custom antibody production

 
 
   
 

Schedule

Time

Cost

 
 

Phase 1

10-12 weeks

2 100 €

 
  Immunization and boosts (4-5 times) of 4 Balb/c mice.      
  Evaluation of titre using ELISA.      
  Micro-isolation of mouse IgG prior to cell-fusion      
  Design and peptide synthesis at an additional cost of 400-650 €.      
 

Phase 2

3-4 weeks

2 900 €

 
  Hybridoma fusion with the spleenocytes obtained from the mouse with the best titer, and myeloma cells. 1-2 fusions are included.      
  Supernatants from the hybridoma clones will be screened by ELISA and supernatants from up to 10 positive clones, will be sent to customer's lab for evaluation. Shipment overseas at additional cost.      
 

Phase 3

4-5 weeks

2 100 €

 
  The customer selects the clones of interest to proceed with. Subcloning of 3 clones is included.      
 

Additional services

     
  Protein A/G purification and expansion (1 litre) per clone   1 100 €  
  Screening with more than one antigen in phase 2   1 000 €  
  Subcloning of hybridom generated from screening with additional antigen (see above).   950 €  
  Third or additional fusions   1 800 €  
  Additional positive clones (>10)   35 €  
  Subcloning of additional positive clones (>3)   350 €  
  Mouse housing charges (per month)   35 €  
  Western strip blotting secondary screening (per 12 samples)   700 €  
  Dot blot screening (per 12 samples)   350 €  
  Labelling of primary antibodies (HRP, Biotin, FITC, frequently used fluorescent dyes)   Price on request  
   
 
SwedClone | Uminova Science Park | Box 7964 | SE-907 19 Umeå, Sweden | Phone: +46 90 15 49 40 / +46 70 655 9440 | E-mail: info@swedclone.com
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